ONE-STEP REVERSE TRANSCRIPTASE PCR Kit is specifically designed for conventional PCR using direct RNA as template. Single-direction control of reverse transcription and PCR using gene-specific primers. Combining the superior performance of Reverse Transcriptase (RT) and Hot Start Champagne Taq DNA Polymerase with an optimized buffering system, the measurement weight of the ONE-STEP REVERSE TRANSCRIPTASE PCR Kit is < 10 copies of RNA template or 0.1 pg total RNA. The ingredients in the kit include premixes, an optimized buffer, dNTP Blend, RNase inhibitor, reverse transcriptase and hot start champagne taq DNA polymerase enzyme blends